Troubleshooting - Non-specific staining
Immunohistochemistry (IHC) troubleshooting for no staining, high background and non-specific staining.
| Problems | Possible reasons | Solutions |
|---|---|---|
| High background | Insufficient washing after antibody incubation | Wash with PBS appropriately |
| Antibody concentration was too high | Decrease the antibody concentration | |
| Presence of endogenous peroxidase | Block endogenous peroxidase activity properly | |
| The secondary antibody is as the same species as the tissue's origin | Change the secondary antibody |
Learn more about:
- Immunohistochemistry Troubleshooting-No Staining
- Immunohistochemistry Troubleshooting-High Background
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- Immunohistochemistry (IHC) protocol
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